NanoQuantum Products

Example ICy and BrCy Near-Infrared Dyes

Dye Name
ICy-86
ICy-90
ICy-97
ICy105
BrCy106
BrCy-10P-107 (BrCy107)

None of these dyes contain metals

Alternate binding chemistry can potentially be prepared as a special request. COOH and NHS esters are generally available.

NQS has also developed other dyes that may be available for specific reporter or photosensitizer requirements. Help us understand your special requirements for higher-mass, small-molecule, high-QY, and high-stability near-IR dyes.

Spectral Data

Spectral data of free dyes characterize their behavior when bound to low-molecular-weight biomolecules such as amino acids, peptides, and oligonucleotides while the data for dye-IgG conjugates characterize behavior of the dyes when bound to high-molecular-weight biomolecules such as proteins and antibodies.

Spectral and physical characteristics of the example product dyes1

Dye Name Conjugate
(D/P)2
Solvent
System3
Absorption
max.
[nm]
Extinction
Coefficient
(ε)
[M–1⋅cm–1]
Emission
max.
[nm]
Quantum
Yield
F)
[%]
Cy 7 PB 7.4 747 200000 776 13
Cy 7 IgG (1.0) PB 7.4 ~750 ~776 4
ICy-86 PB 7.4 721, 760 100000 786 0.6
ICy-86 MeOH 765 200000 795 44
ICy-86 IgG (1.0) PB 7.4 783 796 0.4
ICy-90 PB 7.4 751 270 778 27
ICy-90 IgG (1.0) PB 7.4 757 778 6
ICy-97 MeOH 764 200000 795 44
ICy-105 PB 7.4 750 227300 779 15
ICy-105 IgG (1.0) PB 7.4 755 777 5
BrCy-106 PB 7.4 747 265500 775 18
BrCy-106 IgG (1.0) PB 7.4 751   775 8
BrCy-10P-107 PB 7.4 751 238000 779 20
BrCy-10P-107 IgG (1.0) PB 7.4 754 779 9

1 The best reporters are highlighted with yellow.
2 D/P — dye-to-protein ratio
3 PB 7.4 — phosphate buffer pH 7.4

 

Relative Photostability

NanoQuantum: Dye to Protein Ratio (Figure 1)

Fig. 1. Quantum yield of dye-IgG conjugates vs. dye-to-protein ratio in phosphate buffer (pH 7.4)

NanoQuantum Dye to Protein Ratio (Figure 2)

Fig. 2. Brightness (ΦF × εmax × D/P) of dye-IgG conjugates when excited at the peak absorption
vs. dye-to-protein ratio (D/P) in phosphate buffer (pH 7.4)


The above data characterizes the photochemical behavior of selected NQS dyes bound to high-molecular-weight biomolecules such as proteins and antibodies using Cy 7 as a reference for comparison.

Cy 7 was selected as a reference because it functions in a similar wavelength range and is well known.

Example Photometric Characteristics of Selected Fluorescent Reporters

NanoQuantum: Absorption and Emission

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